Methods Tissue specimens and DNA extraction Blood

Methods Tissue specimens and DNA extraction Blood /www.selleckchem.com/products/ON-01910.html BIIB057 samples were collected at the Fourth Hospital of Hebei University from 66 ESCC patients who underwent esophageal cancer resection in the Department of Thoracic Surgery between 2003 and 2004. The patients were selected when they received endoscopy examination and specimen were confirmed as ESCC by pathologist. All the patients comes from the Hebei Province of China a high risk area of ESCC. The tumor-free controls as determined per endoscopy, radiograph, and blood examination, were randomly selected from the same area. Both patients and controls contain 42 males and 24 females with the mean age of 59.78 ± 8.32 in ESCC

patients and 60.84 ± 8.77 in controls. Genomic DNA was extracted immediately with a Wizard Genomic DNA extraction kit (Promega,

Madison, WI) from blood samples. The study was approved by the Human Tissue Research Committee of the Fourth Hospital of Hebei Medical University. All patients provided written informed consent for the collection of samples and subsequent analysis. PCR amplification and sequence analysis The forward primer 5′-CCCCATGCTTACAAGCAAGT-3′ (nucleotide 16190-16209) and reverse primer 5′-GCTTTGAGGAGGTAAGCTAC-3′ (nucleotide BMS202 in vivo 602-583) were used for amplification of a 982 bp product from mtDNA D-Loop region as described previously [15]. PCR was performed according to the protocol of PCR Master Mix Kit (Promega, Madison, WI) and purified prior to sequencing. Cycle sequencing

was carried out with the Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystem, Foster City, CA) and the products were then separated on the ABIPRISM Genetic Analyzer 3100 (Applied Biosystem). Polymorphisms were confirmed by repeated analyses from both strands. SNPs were identified directly from blood mitochondria. Statistical analysis The χ2 test was used to analyze dichotomous values, such as the presence or absence of an individual SNP between ESCC patients and healthy (-)-p-Bromotetramisole Oxalate controls. The survival curve was calculated using the Kaplan-Meier method, and compared with the log-rank test. Multivariate survival analysis was performed using a Cox proportional hazards model. All of the statistical analysis was done with the SPSS 11.5 software package (SPSS Company, Chicago, IL). A p value of < 0.05 was considered statistically significant. Results A total of 66 patients were enrolled in this study. Six of these patients were lost to follow-up. A review was conducted every six months over a five-year period. Those patients lost to follow-up during this time period were as follows: 1 patient in Year 2; 1 patient in Year 3; 3 patients in Year 4; and, 1 patient in Year 5. Sixty patients shared the same performance status (ECOG Score: Zero).

Comments are closed.