An update on results from pharmacologic and nonpharmacologic prev

An update on results from pharmacologic and nonpharmacologic prevention trials is provided.”
“Objective-To determine the effect of PO administration of pimobendan on clinical

and echocardiographic variables and survival time in cats with heart failure characterized by ventricular systolic dysfunction.

Design-Retrospective cohort study.

Animals-27 client-owned cats (16 male and 11 female) with heart failure, treated with pimobendan (mean +/- SD dosage, 0.26 +/- 0.08 mg/kg [0.118 +/- 0.036 mg/lb], PO, q 12 h).

Procedures-Information on medical history, laboratory results, diagnostic imaging findings, treatments received, and survival time were obtained from medical records of cats that received pimobendan because of cardiac disease. When possible, additional

follow-up information was obtained through telephone interviews with referring veterinarians and owners.

Results-The mean +/- SD age of all 27 cats was 8.9 +/- 5.2 years. All cats had received several cardiac medications. Types of heart disease represented included unclassified cardiomyopathy (CM; n = 11 [41%]), dilated CM (8 [30%]), arrhythmogenic right ventricular CM (4 [15%]), congenital heart disease (3 [11%]), and hypertrophic CM with regional hypokinesis (1 [4%]). All cats had ventricular systolic dysfunction. One cat with systolic anterior motion of the mitral valve became severely hypotensive after initial administration of pimobendan and was excluded from the survival analysis. Median survival time was 167 days (95% confidence interval, 32 to 339 days).

Conclusions and Clinical Relevance-Pimobendan appeared to be well tolerated in cats with heart failure characterized by ventricular

systolic dysfunction of various etiologies. Cats with systolic anterior motion of the mitral valve may develop systemic hypotension when treated with pimobendan. Additional studies are needed to establish dosages for pimobendan and its effects before it can be recommended for treatment of cats with CHF. (J Am Vet MEd Assoc 2012;241:89-94)”
“Polyamines are key regulators of cell development and many plant responses to environmental challenges, however, their functions still remain unclear in complex interactions with other hormones and in biotic or abiotic stress. This lack of knowledge derives from the difficulties on measuring natural polyamines in plants. Here, we present a fast multiresidue method for putrescine (Put), 1,3-diaminopropane (DAP), L-ornithine, spermidine (Spd) and spermine (Spn) measurements in plant samples. Polyamine determination is based on a perchloric acid extraction followed by a simple filtration procedure without previous derivatization. Polyamines are resolved by HPLC in a C18 common column and quantified by electrospray ionization tandem mass spectrometry. (13)C(4)-putrescine and 1,7-diaminoheptane standards were added prior to sample extraction to achieve an accurate quantification in a single run.

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