3072752]”
“Background: DNA methylation changes are potential pathways of environmentally induced health effects. SB202190 We investigated whether exposure to ambient concentrations of NO2, PM10, PM2.5 and O-3 and traffic parameters were associated with global DNA methylation in blood of healthy adults.
Methods: 48 non-smoking adults (25 males) with a median age of 39 years were sampled in winter and summer. Global DNA methylation in whole blood (% 5-methyl-2′-deoxycytidine, %5mdC) was analyzed with HPLC. Exposure to air pollutants at the home address was assessed using interpolated NO2, PM10, PM2.5 and O-3 concentrations for
various exposure windows (60- to 1-day moving average exposures and yearly averages) and GIS-based traffic parameters. Associations between pollutants and %5mdC were tested with multiple mixed effects regression models.
Results: Average %5mdC (SD) was 4.30 (0.08) in winter and 4.29 (0.08) in summer. Men had higher %5mdC compared check details to women both in winter (4.32 vs. 4.26) and summer (431 vs. 4.27). When winter and summer data were analyzed together, various NO2, PM10 and PM2.5 moving average exposures were associated with changes in %5mdC (95% CI) ranging from -0.04 (-0.09
to 0.00) to -0.14 (-0.28 to 0.00) per IQR increase in pollutant. NO2, PM10, PM2.5 and 03 moving average exposures were associated with decreased %5mdC (95% Cl) varying between -0.01 (-0.03 to 0.00) and -0.17 (-0.27 to -0.06) per IQR increase in pollutant in summer but not in winter.
Conclusion: Decreased global DNA methylation in whole blood was associated with exposure to NO2, PM,,,, PM2.5 and O-3 at the home addresses of non- adults. Most effects were observed for the 5- to 30-day
moving average exposures. (C) 2013 Elsevier Ltd. All rights reserved.”
“Background: Improved understanding and advances in treatment regimens have led to increased longevity among patients diagnosed with BKM120 extremity soft tissue sarcomas. Limb salvage techniques and implants have improved and continue to evolve to accommodate the increasing demands and survival of these patients.
Methods: The current report is a review of the literature for recent advancements in techniques, implant design, and outcomes in the field of limb salvage therapy using segmental megaprostheses for the treatment of extremity sarcomas. We report on our experience in this field utilizing a classification system of failure mechanisms to outline to discuss current controversies in management.
Results: Five mechanisms of failure have been identified: soft-tissue failure, aseptic loosening, structural failure, infection, and tumor progression. Infection was the most common mode of failure in our series, accounting for 34% of cases. Soft-tissue failure occurred most commonly in the joints that depend heavily on periarticular muscles and ligaments for stability due to their high degree of functional range of motion.